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Using HEK-293 adherent cells and K-562 suspension cells treated with varying concentrations of menadione or l-Buthionine Sulfoximine (BSO), two compounds known to affect total glutathione (GSH + GSSG) levels, we assayed the cells in separate assays and in a multiplex assay

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Loss of mGPDH sensitized cells to GPX4 inhibitor-induced ferroptosis, whereas supplementation with G3P rescued this vulnerability in a mGPDH-dependent manner

Research Insights DSIP is a small peptide composed of nine amino acids, making it a nonapeptide ( 10 )